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p elk1 antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p elk1 antibody
    P Elk1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+elk1/Elk-1+Antibody/10__1016_slash_j__mtbio__2026__103059-82-21-45
    Average 94 stars, based on 362 article reviews
    p elk1 antibody - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Western Blot:

    Article Title: Viral replication probably promoted by EV71 VP4 protein via activating the PI3K/AKT and MAPK pathways in RD cell
    Article Snippet: In RD cells, after ITGA5 lentivirus infected for 48 h and infected with EV71 (MOI = 0.1, detection by TCID50) for 24 h or 48 h, cells were collected for Western blot and RT-qPCR. .. In this part, proteins level of p-AKT, p-MEK1, and p-Elk1 (CST, USA) in PI3K/AKT or MAPK pathway were detected by WB. ..

    Article Title: Inhibition of importin-7 attenuates ventilator-induced lung injury by targeting nuclear translocation of p38
    Article Snippet: The protein content of cell lysates was determined by bicinchoninic acid (BCA) assay (Beyotime, Shanghai, China). .. For Western blotting, equal amounts of proteins were separated by SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes followed by immunostaining with primary antibody specific for p38 (1:1000), phosphorylated p38 (p-p38) (1:1000), ATF-2 (1:1000), p-ATF-2 (1:1000), p-MK2 (1:1000), p-Elk1 (1:1000) (Cell Signaling Technology, Danvers, MA, USA), and Imp7 (1:1000, Abcam, Cambridge, MA, USA). .. A horseradish peroxidase-conjugated secondary antibody (1:2000, Pierce, Rockford, IL, USA) was then used in a standard enhanced chemiluminescence reaction according to the manufacturer’s instructions.

    Incubation:

    Article Title: Inhibition of importin-7 attenuates ventilator-induced lung injury by targeting nuclear translocation of p38
    Article Snippet: .. The samples were then incubated with primary antibody specific for p38 (1:500), p-p38 (1:200), p-MK2 (1:200), and p-Elk1 (1:200) (Cell Signaling Technology, Danvers, MA, USA), Imp7 (1:200, Thermo Fisher Scientific, Rockford, IL, USA), and F-actin (1:500, Invitrogen, San Diego, CA, USA). .. Samples were washed with PBS containing 0.5% BSA followed by incubation in appropriate Cy3 (1:1000, Invitrogen, Carlsbad, CA, USA) and Cy5 (1:1000, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) conjugated secondary antibodies, and then stained with 100 ng/ml DAPI (Sigma-Aldrich, St. Louis, MO, USA) to visualize nuclei.

    Article Title: Oestrogen receptor α regulates the odonto/osteogenic differentiation of stem cells from apical papilla via ERK and JNK MAPK pathways
    Article Snippet: The same amount of protein was subject to a 10% sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to polyvinylidene fluoride (PVDF, Millipore, Darmstadt, Germany) membranes. .. The transblotted membranes were blocked in 5% BSA for 2 hours at room temperature followed by an incubation with primary antibodies including DMP1 (NBP1‐45525, Novus, Littleton, USA), RUNX2 (ab76956, Abcam, Cambridge, UK), DSP (sc‐33586, Santa Cruz), OCN (ab93876, Abcam), ERK (#4695, Cell Signaling Technology), p‐ERK (#4370, Cell Signaling Technology, Danvers, MA, USA), JNK (#9252, Cell Signaling Technology), p‐JNK (#9255, Cell Signaling Technology), P38 (#8690, Cell Signaling Technology), p‐P38 (#4511, Cell Signaling Technology), c‐Fos (#2250, Cell Signaling Technology), p‐c‐Fos (#5348, Cell Signaling Technology), c‐Jun (#9165, Cell Signaling Technology), p‐c‐Jun (#9261, Cell Signaling Technology), Elk1 (#9182, Cell Signaling Technology), p‐Elk1 (#9186, Cell Signaling Technology), H3 (#9728, Cell Signaling Technology) and β‐ACTIN (AP0060, Bioworld, Nanjing, China) at 4°C overnight. ..

    Membrane:

    Article Title: Microtubule Acetylation-Specific Inhibitors Induce Cell Death and Mitotic Arrest via JNK/AP-1 Activation in Triple-Negative Breast Cancer Cells
    Article Snippet: Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer composed of 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.1% SDS, 10 mM NaF, 1 mM Na3VO4, and protease inhibitor cocktail (Roche, Switzerland). .. The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Merck, Germany) and detected using antibodies against acetyl-α-tubulin (T6199; Sigma), p-JNK (4668; Cell Signaling 3 Microtubule Acetylation/JNK/AP-1 Axis in TNBC Suyeon Ahn et al. Technology [CST], USA), JNK (AHO1362; Invitrogen, USA), p-ERK (9106; CST), ERK (4696; CST), p-p38 (9215; CST), p38 (9212; CST), p-c-Jun (3270; CST), c-Jun (ab32137; Abcam, UK), c-Fos (2250; CST), p-ATF2 (27934; CST), ATF2 (35031; CST), Bcl2 (4223; CST), p-Histone H3 (9701; CST), p-cdc2 (4539; CST), p-Elk1 (9181; CST), α-tubulin (T6199; Sigma), and cyclin antibody sampler kit (9869; CST). .. Total RNA was isolated from cells using TRIzol (Invitrogen) according to the manufacturer’s protocol, and mRNA was reverse transcribed into cDNA using TOPscript RT DryMIX (Enzynomics, Korea).

    Article Title: Microtubule Acetylation-Specific Inhibitors Induce Cell Death and Mitotic Arrest via JNK/AP-1 Activation in Triple-Negative Breast Cancer Cells
    Article Snippet: Cells were lysed with radioimmunoprecipitation assay (RIPA) buffer composed of 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.1% SDS, 10 mM NaF, 1 mM Na3VO4, and protease inhibitor cocktail (Roche, Switzerland). .. The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Merck, Germany) and detected using antibodies against acetyl-α-tubulin (T6199; Sigma), p-JNK (4668; Cell Signaling Technology [CST], USA), JNK (AHO1362; Invitrogen, USA), p-ERK (9106; CST), ERK (4696; CST), p-p38 (9215; CST), p38 (9212; CST), p-c-Jun (3270; CST), c-Jun (ab32137; Abcam, UK), c-Fos (2250; CST), p-ATF2 (27934; CST), ATF2 (35031; CST), Bcl2 (4223; CST), p-Histone H3 (9701; CST), p-cdc2 (4539; CST), p-Elk1 (9181; CST), α-tubulin (T6199; Sigma), and cyclin antibody sampler kit (9869; CST). .. Total RNA was isolated from cells using TRIzol (Invitrogen) according to the manufacturer’s protocol, and mRNA was reverse transcribed into cDNA using TOPscript RT DryMIX (Enzynomics, Korea).

    SDS Page:

    Article Title: Inhibition of importin-7 attenuates ventilator-induced lung injury by targeting nuclear translocation of p38
    Article Snippet: The protein content of cell lysates was determined by bicinchoninic acid (BCA) assay (Beyotime, Shanghai, China). .. For Western blotting, equal amounts of proteins were separated by SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes followed by immunostaining with primary antibody specific for p38 (1:1000), phosphorylated p38 (p-p38) (1:1000), ATF-2 (1:1000), p-ATF-2 (1:1000), p-MK2 (1:1000), p-Elk1 (1:1000) (Cell Signaling Technology, Danvers, MA, USA), and Imp7 (1:1000, Abcam, Cambridge, MA, USA). .. A horseradish peroxidase-conjugated secondary antibody (1:2000, Pierce, Rockford, IL, USA) was then used in a standard enhanced chemiluminescence reaction according to the manufacturer’s instructions.

    Nucleic Acid Electrophoresis:

    Article Title: Inhibition of importin-7 attenuates ventilator-induced lung injury by targeting nuclear translocation of p38
    Article Snippet: The protein content of cell lysates was determined by bicinchoninic acid (BCA) assay (Beyotime, Shanghai, China). .. For Western blotting, equal amounts of proteins were separated by SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes followed by immunostaining with primary antibody specific for p38 (1:1000), phosphorylated p38 (p-p38) (1:1000), ATF-2 (1:1000), p-ATF-2 (1:1000), p-MK2 (1:1000), p-Elk1 (1:1000) (Cell Signaling Technology, Danvers, MA, USA), and Imp7 (1:1000, Abcam, Cambridge, MA, USA). .. A horseradish peroxidase-conjugated secondary antibody (1:2000, Pierce, Rockford, IL, USA) was then used in a standard enhanced chemiluminescence reaction according to the manufacturer’s instructions.

    Immunostaining:

    Article Title: Inhibition of importin-7 attenuates ventilator-induced lung injury by targeting nuclear translocation of p38
    Article Snippet: The protein content of cell lysates was determined by bicinchoninic acid (BCA) assay (Beyotime, Shanghai, China). .. For Western blotting, equal amounts of proteins were separated by SDS-PAGE gel electrophoresis and transferred to nitrocellulose membranes followed by immunostaining with primary antibody specific for p38 (1:1000), phosphorylated p38 (p-p38) (1:1000), ATF-2 (1:1000), p-ATF-2 (1:1000), p-MK2 (1:1000), p-Elk1 (1:1000) (Cell Signaling Technology, Danvers, MA, USA), and Imp7 (1:1000, Abcam, Cambridge, MA, USA). .. A horseradish peroxidase-conjugated secondary antibody (1:2000, Pierce, Rockford, IL, USA) was then used in a standard enhanced chemiluminescence reaction according to the manufacturer’s instructions.



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